10X MOPS Buffer |
| 3-(N-morpholino)-propane sulfonic acid | 40 g per Liter |
| Sodium Acetate | 2 g per Liter |
| 500 mM EDTA (autoclaved) | 20 mL per Liter |
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Dissolve components in 980 mL deionized water, pH to 7.0 with NaOH (takes several pasteur pipettes worth of 10 N). QS to 1 Liter with deionized water. Filter. Do not autoclave MOPS. Wear gloves when preparing. Wrap bottle in aluminum foil. Store at 4C. |
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RNA Sample Buffer (per mL) |
| Formamide | 562 uL |
| Formaldehyde (37%) | 187 uL |
| 10x MOPS | 112 uL |
| ddH2O | 128 uL |
| Ethidium Bromide (10 mg/mL) | 11 uL |
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Make about 10 mL and aliquot 500 uL portions into eppendorfs. Store at -80C. Treat as 2X. There is special store of EtBr for use with RNA. |
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RNA Formaldehyde/Agarose Gel |
| Rnase free agarose | 1.2 g |
| ddH2O | 85 mL |
| 10X MOPS | 10 mL |
| Formaldehyde (37%) | 5 mL |
| Microwave agarose and water. Cool to 60C, then add MOPS and formaldehyde in the hood. Pour gel. |
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DEPC Water (per mL) |
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Add 1 mL diethyl pyrocarbonate to 1 Liter of distilled water.
Shake vigorously to get the DEPC into solution.
Allow solution to stand for 5-6 hours
Autoclave (liquid, 20 minutes)
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