Transcriptome analysis was performed during differentiation of G1E cells, a murine cell line which recapitulates erythropoiesis from the late CFU-E through the basophilic erythroblast stage
(Weiss et al., 1997). G1E cells were derived by targeted gene deletion of the single copy of GATA-1 on the X chromosome . GATA-1 is a "master regulator" zinc finger transcription factor which directs erythropoiesis (
Weiss and Orkin, 1995
). It is found in the promoter of almost all genes which contribute to the erythroid phenotype, and mutations of GATA-1 result in anemia and thrombocytopenia . The analysis was performed using Affymetrix GeneChips, and the microarray data have been deposited with the
NIH GEO database, accession
GSE628. The results are summarized in Welch et al., 2003. and compiled in the G1EDb database.