Up-coming Seminars

• Dec 1 - David Feldheim ... 12:00 PM - CHOP

Transgenic Core (internal link)
The Transgenic Core Facility affords scientists the opportunity to genetically manipulate the mouse genome in an effort to better model and study human disease. Services offered by the Transgenic Core include transgenic and knockout/in mouse production, mouse line rederivation, embryo and sperm cryopreservation and in vitro fertilization. The facility is located within the semi-barrier unit of the Laboratory Animal Facility (LAF), where use of pathogen-free provisions enable mice to be transferred to any other holding room within the LAF. For further information contact the facility's technical director, Adele Button, at 267-426-5836, 215-590-5753 or button@email.chop.edu. Gene Targeting Core

Gene Targeting Core (internal link)
The Gene Targeting Core Facility provides complete services for generating targeted mutant and transgenic mice. Located in the Abramson Research Center, Room 814L, the core currently consists of a general lab and tissue culture room. The core offers a number of services: embryonic stem cell targeting, clonal expansion, karyotypic analysis, and targeted clone selection and preparation for microinjection. For further information contact Rachel Martin at 267-426-2773 or martinr@email.chop.edu.

Pathology Core
Located in Abramson 706, the Pathology Core unites three core components: histopathology, TMA/LCM and confocal microscopy. The core offers a full range of histopathology services, including tissue processing, embedding and cutting for both paraffin and frozen tissue. The core also performs most standard stains as well as immunohistochemistry, antibody workup, fluorescence and in situ hybridization. TMAs are constructed using a Beacher Semi-automated Arrayer. Sophisticated imaging instrumentation is available for virtual microscopy and conducting Web-based data analysis. LCM is performed with a Zeiss/P.A.L.M. instrument capable of capture from both glass slides and tissue cultures. A Leica spectral confocal system uses an acoustical-optical tunable filter for use of up to seven lasers lines, including UV. This state-of-the-art confocal microscope permits 3-D fluorescent imaging of both live and fixed specimens, and has capabilities for FRET and FRAP imaging.

Mass Spectrometry Core
The Mass Spectrometry Core Facility provides state-of-the-art services to investigators, with a special emphasis on the determination of stable isotope enrichment for studies of metabolite turnover. The facility includes multiple mass spectrometry systems with a variety of capabilities including an electrospray mass spectrometer with a molecular weight range of 50-30,000 daltons. Primary systems for analytical mass spectrometry are GC/MS, which have a molecular weight range of 10-500 daltons. We have recently added a gas ratio mass spectrometer, which is used to quantitate isotopic ratios of a variety of gases.

Nucleic Acid Protein Core (internal link)
The Nucleic Acid/Protein Core Facility provides numerous services in support of investigators' molecular biology needs. These services include DNA sequencing, gene mapping, detection of mutations and polymorphisms, genotyping, RNA analysis and gene expression profiling with microarrays. For further information, please contact Dr. Zhili Wang (wangz@email.chop.edu) or Dr. Eric Rappaport (Rappaport@email.chop.edu) at 215-590-3897.

Protein Core
The Hospital's Protein Core Facility, opened in September 2003, provides a variety of complementary techniques that enable CHOP researchers to produce and characterize proteins and investigate the protein-protein interactions. The core facility is located at 806 ARC and features four modules: protein expression, protein analysis, protein-protein interactions and proteomics. Protein expression services include recombinant protein production in both bacteria and insect cells production of established monoclonal antibodies as well as peptides. HPLC and FPLC equipment, columns and resins are available for a variety of purification needs. The protein analysis module provides analytical capabilities to characterize purified proteins, determine their folding status and their ability to bind small molecule ligands, using endogenous fluorescence, circular dichroism and analytical centrifugation. The protein-protein interactions module provides affinity chromatography, analytical centrifugation and a SELDI mass spectrometer. We can also help with surface plasmon resonance and isothermal calorimetry experiments. The proteomics module includes fractionation of complex protein mixtures and identification of proteins, using multi-dimensional liquid chromatography, MALDI-TOF analysis or MS/MS identification of individual proteins as well as ICAT analysis of peptides and database searches. Some services are provided on a user-operated, sign-up basis, while others are performed as full-service by the facility personnel. For initial consultation and help with meeting your research needs contact the facility's director, Dr. Michael Rosenblatt (rosenblatt@email.chop.edu).

Real-Time PCR Core (internal link)
The Real-Time PCR Core Facility provides cutting-edge real time quantitative PCR capabilities to the research community. Its new ABI Prism 7900HT with automation accessory is capable of higher throughput than the original SDS-7700 and the newer SDS-7000 "TaqMan" systems previously available to CHOP investigators. The new machine can offer 96wells, 384 wells and the latest micro fluidic cards applications from Applied Biosystems, while the older machines can handle up to 96 wells per run. The core offers support in terms of training, experimental designs, data analysis and trouble shooting.