Stokes Research Core

Stocked Items

PCF Common Stocks

Chromatography Columns - Ciphergen Protein Chips - Plasmids and Transfer Vectors

The PCF routinely stocks commonly needed chromatography and desalting reagents. Additionally, we also stock various transfer vectors and plasmids for bacterial expression. Below is a partial listing of our available stock.

Chromatography Columns
HiTrap SP HP
HiTrap DEAE FF
HiTrap IEX Selection
HiTrap CM FF
HiTrap SP FF
HiTrap Q HP
HiTrap Chelating HP
NHS-activated sepharose 4 FF
HiTrap r Protein A
Source 15S
Source 15Q
CM Sepharose FF
DEAE Sepharose FF
SP Sepharose FF
Q Sepharose FF
GST Prep FF 16/10
Calmodulin Sepharose 4B
Streptaviding Sepharose HP
rProtein A Sepharose FF
HiPrep 26/10 Desalting
Glutathione Sepharose 4 FF
Mono Q 5/50 GL
Mono S HR 5/5
HiPrep 26/60 Sephacryl S-100HR
HiPrep 26/60 Sephacryl S-200HR
HiPrep 26/60 Sephacryl S-300HR
Superdex Peptide 10/300 GL
Mono P 5/200 GL
Superdex 200 10/300 GL

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Ciphergen Protein Chips
Gold Chip
SAX2 Chip
WCX2 Chip
PS10 Chip
PS20 Chip
Q10 Chip
IMAC3 Chip
IMAC30 Chip
H4 Chip
H50 Chip
CM10 Chip
NP20 Chip
RS100

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Vectors available at the PCF

Vector name	Features	Expression Host	Vendor
pENTR3C	Gateway pENTR vector	N/A	Invitrogen
pENTR2B	Gateway pENTR vector	N/A	Invitrogen
pENTR1A	Gateway pENTR vector	N/A	Invitrogen
pENTR4	Gateway pENTR vector	N/A	Invitrogen
pDONR221	Gateway pENTR vector	N/A	Invitrogen
pDEST10	Polyhedrin promotor, N-term 6XHis	Baculovirus	Invitrogen
pDEST15	N-term GST	E. coli	Invitrogen
pDEST17	N-term 6xHis	E. coli	Invitrogen
pDEST20	Polyhedrin promotor, N-term GST	Baculovirus	Invitrogen
pDEST42	C-term V5-6xHis	E. coli	Invitrogen
pAcGHLT-A	transfer vector, N-term GST, 6xHis, thrombin cleavage site	Baculovirus	BD Biosicences
pAcGHLT-B	transfer vector, N-term GST, 6xHis, thrombin cleavage site	Baculovirus	BD Biosicences
pAcGHLT-C	transfer vector, N-term GST, 6xHis, thrombin cleveage site	Baculovirus	BD Biosicences
pAcUW51	transfer vector, Polyhedrin and p10 promotors, express 2 foreign genes	Baculovirus	BD Biosicences
pAcG3X	transfer vector, N-term GST, factor Xa cleavage site	Baculovirus	BD Biosicences
pAcMp2	transfer vector, facilitates post-translational modifications	Baculovirus	BD Biosicences
pAcMP3	transfer vector, facilitates post-translational modifications	Baculovirus	BD Biosicences
pAcSecG2T	transfer vector, polyhedrin promotor, signal sequence, GST-tag	Baculovirus	BD Biosicences
pFastBacHTa	Invitrogen Bac-to-Bac Baculovirus system, N-term 6xHis , TEV cleavage site	Baculovirus	Invitrogen
pFastBacHTb	Invitrogen Bac-to-Bac Baculovirus system, N-term 6xHis , TEV cleavage site	Baculovirus	Invitrogen
pFastBacHTc	Invitrogen Bac-to-Bac Baculovirus system, N-term 6xHis , TEV cleavage site	Baculovirus	Invitrogen
BaculoDirect C-term linear DNA	C-termV5-6XHis	Baculovirus	Invitrogen
pET-41b(+)	GST, 6xHis, S tags,	E. coli	Novagen
pET-25b(+)	C-term HSV and His, signal sequence, EK cleavage site, allow to have generation of native proteins with their authentic N-term	E. coli	Novagen
pET-32a(+)	6xHis, Trx, and S tags	E. coli	Novagen
pET-16b	N-term 6xHis, Factor Xa cleavage site	E. coli	Novagen
pQE30Xa	T5 promoter, N-term His tag, Factor Xa cleavage site	E. coli	Qiagen

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