Our Mission
The Pathology Core Laboratory at the Joseph Stokes Jr. Research Institute provides basic histopathology, research immunohistochemistry, tissue microarray, laser capture microdissection and confocal microscopy services to researchers at Children's Hospital and within the surrounding academic community. We are located on the 7th Floor of the Leonard and Madlyn Abramson Pediatric Research Center in room 706. Daniel Martinez 267-426-5635 martinezd@email.chop.edu is the Lab Manager and can address all questions regarding basic histopathology, research immunohistochemistry, tissue microarray and laser capture microdissection. Questions regarding access to confocal microscopy and other advanced micoscopic imaging services should be directed to Ed Williamson at 215-590-3390, williamsone@email.chop.edu. Dr. Alexander R. Judkins, M.D. is the Faculty Director.
The Pathology Core Laboratory unites three core components in a single core facility: histopathology, tissue microarray and confocal microscopy. The core offers a full range of histopathology services including tissue processing, embedding, and cutting, for both paraffin and frozen tissue. We also perform most standard stains as well as immunohistochemistry, antibody workup, fluorescence, in situ hybridization and TUNEL. Tissue microarrays can be constructed using a Beacher Arrayer. Sophisticated imaging instrumentation is available for virtual microscopy (ScanScope from Aperio) and image analysis (Imag ProPlus, Volocity). Specialized software is available to image and analyze tissue microarrays, and to manage and store array data.
Confocal microscopy is an essential microscopic imaging tool for scientific research. The advantages of confocal microscopy compared to conventional microscopes are better resolution, clearer images and greater sensitivity. The Leica DM IRE2 spectral confocal system uses an acoustical-optical tunable filter for use of up to 7 lasers lines, including UV. This state-of-the-art confocal microscope permits 3D fluorescent imaging of both live and fixed specimens, and has capabilities for FRET and FRAP imaging. With Z-axis scanning, our instrument can produce a three dimensional view of specimens to better visualize intracellular organelles and structures. Software for analysis and 3D rendering of images is also available.
In addition to conventional approaches based on labeling fixed cells with fluorescently-tagged antibodies, a wide array of fluorescent dyes and physiological indicators is now available that can be used to track organelle movement in living cells or probe specific changes in cell/organelle metabolism in response to environmental stimuli. The spectral imaging capabilities of our microscope permit full use of these reagents, and our facility staff are prepared to assist users with all steps of experimental design, data acquisition and analysis.