Our Mission
The Pathology Core at the Stokes Institute provides basic histopathology, tissue microarray and confocal services to researchers at Children's Hospital and within the surrounding academic community. The core is located on the 7th Floor of the Leonard and Madlyn Abramson Pediatric Research Center in room 706. Questions regarding histochemistry or tissue microarray services should be directed to Daniel Martinez 267-426-5635 martinezd@email.chop.edu. Questions regarding access to confocal microscopy services should be directed to Ed Williamson at 215-590-3390, williamsone@email.chop.edu
The
Pathology Core unites three core components in a single core facility: histopathology,
tissue microarray and confocal microscopy. The core offers a full range of
histopathology services including tissue processing, embedding, and cutting,
for both paraffin and frozen tissue. We also perform most standard stains
as well as immunohistochemistry, antibody workup, fluorescence, in situ hybridization
and TUNEL. Tissue microarrays can be constructed using a Beacher Semi-automated
Arrayer. Sophisticated imaging instrumentation is available for virtual microscopy
(BLISS from Bacus Labs) and conducting web-based data analysis. Specialized
software is available to image and analyze tissue microarrays, and to manage
and store array data. The Leica DM IRE2 spectral confocal system uses an
acoustical-optical tunable filter for use of up to 7 lasers lines, including
UV. This state-of-the-art confocal microscope permits 3D fluorescent imaging
of both live and fixed specimens, and has capabilities for FRET and FRAP
imaging. Software for analysis and 3D rendering of images is also available.