Histopathology Services
The Pathology core supports a full range of basic pathology services. We accept wet fixed tissue specimens as well as frozen samples. Wet samples are processed on one of two automated tissue processors. The processing protocol is adjusted to match the size of the tissue specimen submitted. For instructions on submitting tissue samples please download our tissue submition guidelines:
General:
The Pathology Core has four microtomes and two embedding stations. One set is available for walk in customer use. The core also has two cryostats for cutting frozen sections. There are storage areas for wet tissue, blocks, slides, and antibodies at 4 degrees, -20, and -80.
The Core Lab performs a variety of histochemical stains also known as special stains.
Special Stains:
- Alkaline Congo Red Method (Puchtler Et Al)
- Gallyas Stain Protocol
- H&E Staining Protocol
- Jones' Method
- Kluver-Barrera Method For Myelin And Nerve Cells
- Masson Trichrome Stain
- Movat (Russell-Movat) Pentachrome Stain
- Nassar And Shanklin Reticulin Stain
- Pas Hematoxylin
- Thioflavine-S /Lipofuscin Autofluorescence Quenching Protocol
- Toluidine Blue For Mast Cells
- Tunel Labeling-Roche Protocol
- X-Gal Staining Protocol
Other stains available upon request. Click Here to request other stains.
Five Headed Scope: A Leica DM2500 multiheaded scope allows up to five researchers to view the same slide simultaneously at magnifications up to 630x
Basic Imaging Workstation: A Leica DM4000B upright scope paired with a Spot RT/SE Slider camera provides both brightfield and widefield fluorescence imaging capabilities. Optimized for 100micron coverslips.
Objectives
- 1.25x/N.A. 0.04
- 5x/N.A. 0.15
- 10x/N.A. 0.40
- 20x/N.A. 0.70
- 40x/N.A. 0.85
- 63x/N.A. 0.90
- 60x/N.A. 1.40-0.6 Oil (by appointment only)
- 100x/N.A. 1.40-0.7 Oil (by appointment only)
Filter Cubes
- Leica A4 – DAPI (Blue)
- Leica L5 – FITC (Green)
- Leica TX2- TxRd (Red)
- Leica BGR – Triple Cube
- Leica Y5 – Cy5 (Far Red)
Image Pro provides image analysis tools such as cell counting and percent area stained measurements. The imaging workstation is also equipped with Adobe Photoshop for image "tweaking."
Slide Scanning: Whole slides can be scanned and digitized to create "virtual slides" using the Aperio scanscope. The images are stored on a server making remote access possible from within the CHOP firewall. Slides can be viewed with any internet browser or else by using Image Scope. Image Scope is available free of charge and allows users to remotely conference with other users while looking at the same slides or images.